KMID : 0624620200530010003
|
|
BMB Reports 2020 Volume.53 No. 1 p.3 ~ p.9
|
|
Techniques for investigating mitochondrial gene expression
|
|
Park Dong-Keun
Lee So-Yeon Min Kyung-Tai
|
|
Abstract
|
|
|
The mitochondrial genome encodes 13 proteins that are components of the oxidative phosphorylation system (OXPHOS), suggesting that precise regulation of these genes is crucial for maintaining OXPHOS functions, including ATP production, calcium buffering, cell signaling, ROS production, and apoptosis. Furthermore, heteroplasmy or mis-regulation of gene expression in mitochondria frequently is associated with human mitochondrial diseases. Thus, various approaches have been developed to investigate the roles of genes encoded by the mitochondrial genome. In this review, we will discuss a wide range of techniques available for investigating the mitochondrial genome, mitochondrial transcription, and mitochondrial translation, which provide a useful guide to understanding mitochondrial gene expression.
|
|
KEYWORD
|
|
Mitochondrial DNA, Mitochondrial Protein, Mitochondrial RNA
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|